The term "Colonial Spanish Mustang" or simply "Spanish Mustang" refers to horses descended from Spanish stock brought to the Americas during the 16th and 17th centuries, surviving in isolated populations that retained original Spanish characteristics through limited or no crossbreeding with other horse types. The word "mustang" derives from the Spanish "mestengo" or "mostrenco," meaning stray or feral livestock, though Colonial Spanish Mustangs represent distinct genetic populations rather than generic wild horses. The "Colonial Spanish" designation distinguishes these heritage horses from mixed-breed feral horses often called mustangs but lacking the specific Spanish ancestry and genetic characteristics defining true Colonial Spanish horses.
Several registry organizations document and preserve Colonial Spanish horses, with the Horse of the Americas Registry and Spanish Mustang Registry among the primary organizations maintaining studbooks and breed standards. These registries require genetic testing, conformation evaluation, and documentation of Spanish ancestry for registration, distinguishing Colonial Spanish horses from other mustang populations. Additional strain names describe specific populations or lineages including Sulphur horses, Kiger mustangs, Pryor Mountain horses, and Choctaw horses, each representing genetically distinct Colonial Spanish populations that survived in different geographic regions. While sharing common Spanish ancestry, these strains show slight variations reflecting their isolated development over centuries.
The preservation of Colonial Spanish horses represents important conservation work maintaining genetic resources and historical heritage. These horses carry bloodlines of Spanish breeds including Andalusians, Spanish Jennets (now extinct in Spain), Barbs, and other Iberian types that shaped New World history. Their DNA provides genetic record of 16th-century Spanish horse populations that no longer exist in pure form in their homeland. Understanding the Colonial Spanish Mustang's significance requires recognizing them not simply as wild horses but as living artifacts preserving genetics and characteristics of horses instrumental in European exploration, conquest, and development of the Americas.

